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Agilent |
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| The plot presents clustering data that demonstrates that probes for consensus regions 2 and 3 exhibit similar behavior, while probes for consensus region 1 exhibit different behavior, and thus represent, in this case, a real alternative 3' end of transcript A. |
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| Histogram showing the number of probes on the Whole Human Genome related to their distance from the 3' end of the target transcript. Degraded RNAs generally are detected more effectively with 3' biased probes. |
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Low noise and excellent microarray-to-microarray format reproducibility leads to more consistent data.
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Shown here are results of experiments that determined the data correlation between Agilent's 22K Human Oligo Microarrays and the higher-density 44K Whole Human Microarray format.
Complex human targets were run on each type of microarray and subsequent analysis of the combined GREEN signals and RED signals yield tight single color data correlation from the 22K to 44K microarray formats. Further analysis of the mean log BLUE ratios of the complex human targets show a favorable R2 value of 0.9797 between the two microarray formats. |
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