Affymetrix
The Affymetrix GeneChip® System GCS3000 provides the opportunity for both gene expression profiling and DNA wide analysis. It can be used for linkage analysis, association studies involving of copy number changes, LOH, etc. DNA Resequencing and sequencing of the entire mitochondrial genome are other screening options.
CombiMatrix
The ElectraSense™ Platform is an affordable, user-friendly and reliable microarray reader that electrochemically measures thousands of probes in less than one minute.The CustomArray™ Synthesizer offers researchers the ability to fabricate low, medium and high density microarrays. The open architecture platform is easy to use and enables researchers to synthesize novel applications, build from the CombiMatrix list of pre-designed microarrays, or customize microarrays.
Agilent
The Agilent DNA microarray scanner is a 48-slide scanning system that can read any mix of 1 x 3” glass slide microarrays (both Agilent and non-Agilent) and seamlessly feature extract them all using Agilent’s Feature Extraction Software.;
Illumina
Besides next generation sequencing technology by 454, Creative Genomics has extended its portfolio of genomic services with Solexa technology. Generating high quality readout of one billion bases per run at less than 1% of the cost of capillary-based methods, the Illumina Genome Analyzer is designed to enable researchers to dramatically improve the efficiency of current applications.
1. Identification of CD36 as a new surface marker of marginal zone B cells by transcriptomic analysis. Zhang, P. et al. Molecular Immunology44(4), 332-7, 2007
2. Identification of IRF-8 and IRF-1 target genes in activated macrophages. Dror, N. et al.Molecular Immunology 44(4), 338-46, 2007
2. Validation of a fully integrated microfluidic array device for influenza A subtype identification and sequencing.Anal Chem. 2006 Jun 15;78(12):4184-93. PMID: 16771549
Q: HOW MANY SAMPLES DO I NEED IN MICROARRAY EXPERIMENTS
A: We recommend at least 3 replicates used in expression microarray experiment. As well, considering the individual variations, such as human tissues, the sample used for replicates should be larger.
Q: HOW IS CUSTOMARRAY DIFFERENT FROM SPOTTED ARRAYS?
A: CustomArray is an in situ synthesis technology. Oligo nucleotide probes are synthesized directly on the chip. This means there are no up front costs of buying hundreds of different oligonucleotides, and making changes to chip designs and layouts is easy.
Q: HOW IS CUSTOMARRAY DIFFERENT FROM OTHER IN SITU SYNTHESIS TECHNOLOGIES?
A: CustomArray chips use electrochemical detritylation to control DNA synthesis. Electrochemical detritylation is a much more efficient method to synthesize oligonucleotides than light-based synthesis processes. This means the oligonucleotides are of the highest quality and the sensitivity of the microarray is maximized. Since physical photolithographic masks, mechanical micromirrors, or inkjet technologies are NOT involved in the process, all probes can be easily changed without extra time or costs.
